[HTML][HTML] Identification of enhanced serine kinase activity in insulin resistance
L Qiao, JL Goldberg, JC Russell, XJ Sun - Journal of Biological Chemistry, 1999 - ASBMB
Insulin receptor substrate (IRS) proteins play a crucial role as signaling molecules in insulin
action. Serine phosphorylation of IRS proteins has been hypothesized as a cause of
attenuating insulin signaling. The current study investigated serine kinase activity toward
IRS-1 in several models of insulin resistance. An in vitro kinase assay was developed that
used partially purified cell lysates as a kinase and glutathioneS-transferase fusion proteins
that contained various of IRS-1 fragments as substrates. Elevated serine kinase activity was …
action. Serine phosphorylation of IRS proteins has been hypothesized as a cause of
attenuating insulin signaling. The current study investigated serine kinase activity toward
IRS-1 in several models of insulin resistance. An in vitro kinase assay was developed that
used partially purified cell lysates as a kinase and glutathioneS-transferase fusion proteins
that contained various of IRS-1 fragments as substrates. Elevated serine kinase activity was …