A number of modifications to the standard procedures for coupling of fluorochromes to
antibodies are described. The suggested procedures result in economies of time, labour and
materials, and allow the reliable production of high quality conjugates. The modifications
include the use of staphylococcal protein A-Sepharose for (a) a simple one-step procedure
for preparation of IgG from whole serum,(b) removal of undigested IgG after pepsin
treatment, and (c) concentration of dilute solutions of IgG. Many other details of coupling …