Identification of the ron Gene Product as the Receptor for the Human Macrophage Stimulating Protein

MH Wang, C Ronsin, MC Gesnel, L Coupey, A Skeel… - Science, 1994 - science.org
MH Wang, C Ronsin, MC Gesnel, L Coupey, A Skeel, EJ Leonard, R Breathnach
Science, 1994science.org
Macrophage-stimulating protein (MSP) is a member of the hepatocyte growth factor-scatter
factor (HGF-SF) family. Labeled MSP bound to Madin-Darby canine kidney (MDCK) cells
transfected with complementary DNA encoding Ron, a cell membrane protein tyrosine
kinase. Cross-linking of 125I-labeled MSP to transfected cells (MDCK-RE7 cells) and
immunoprecipitation by antibodies to Ron revealed a 220-kilodalton complex, a size
consistent with that of MSP (80 kilodaltons) cross-linked to the β chain of Ron (150 …
Macrophage-stimulating protein (MSP) is a member of the hepatocyte growth factor-scatter factor (HGF-SF) family. Labeled MSP bound to Madin-Darby canine kidney (MDCK) cells transfected with complementary DNA encoding Ron, a cell membrane protein tyrosine kinase. Cross-linking of 125I-labeled MSP to transfected cells (MDCK-RE7 cells) and immunoprecipitation by antibodies to Ron revealed a 220-kilodalton complex, a size consistent with that of MSP (80 kilodaltons) cross-linked to the β chain of Ron (150 kilodaltons). The binding of 125I-labeled MSP to MDCK-RE7 cells was inhibited by unlabeled MSP, but not by HGF-SF. MSP caused phosphorylation of the β chain of Ron and induced migration of MDCK-RE7 cells. These results establish the ron gene product as a specific cell-surface receptor for MSP.
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