[HTML][HTML] Specific binding of the chemokine platelet factor 4 to heparan sulfate
SE Stringer, JT Gallagher - Journal of Biological Chemistry, 1997 - ASBMB
Platelet factor 4 is a tetrameric heparin binding chemokine released from the α-granules of
activated platelets. In this study we show that platelet factor 4 binds with high affinity and
specificity to an approximately 9-kDa sequence in heparan sulfate, which it protects from
degradation by heparinase enzymes. This protected fragment is enriched in N-sulfated
disaccharides and iduronate 2-O-sulfate residues, the latter being important for binding to
platelet factor 4. The major structural motif of the fragment appears to consist of a pair of …
activated platelets. In this study we show that platelet factor 4 binds with high affinity and
specificity to an approximately 9-kDa sequence in heparan sulfate, which it protects from
degradation by heparinase enzymes. This protected fragment is enriched in N-sulfated
disaccharides and iduronate 2-O-sulfate residues, the latter being important for binding to
platelet factor 4. The major structural motif of the fragment appears to consist of a pair of …