[HTML][HTML] Heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A sulfatesN-unsubstituted glucosamine residues
J Liu, Z Shriver, P Blaiklock, K Yoshida… - Journal of Biological …, 1999 - ASBMB
3-O-Sulfation of glucosamine by heparan sulfate d-glucosaminyl 3-O-sulfotransferase (3-
OST-1) is the key modification in anticoagulant heparan sulfate synthesis. However, the
heparan sulfates modified by 3-OST-2 and 3-OST-3A, isoforms of 3-OST-1, do not have
anticoagulant activity, although these isoforms transfer sulfate to the 3-OH position of
glucosamine residues. In this study, we characterize the substrate specificity of purified 3-
OST-3A at the tetrasaccharide level. The 3-OST-3A enzyme was purified from Sf9 cells …
OST-1) is the key modification in anticoagulant heparan sulfate synthesis. However, the
heparan sulfates modified by 3-OST-2 and 3-OST-3A, isoforms of 3-OST-1, do not have
anticoagulant activity, although these isoforms transfer sulfate to the 3-OH position of
glucosamine residues. In this study, we characterize the substrate specificity of purified 3-
OST-3A at the tetrasaccharide level. The 3-OST-3A enzyme was purified from Sf9 cells …