[HTML][HTML] Triiodothyronine Induces the Transcription of the Uncoupling Protein Gene and Stabilizes Its mRNA in Fetal Rat Brown Adipocyte Primary Cultures (∗)

C Guerra, C Roncero, A Porras, M Fernández… - Journal of Biological …, 1996 - ASBMB
Confluent fetal rat brown adipocytes in primary culture showed an almost undetectable level
of uncoupling protein (UCP) mRNA and a low mitochondrial content of functional UCP.
Treatment of confluent cells with 10 nM triiodothyronine in a serum-free medium, in the
absence of noradrenergic stimulation, increased the amount of UCP mRNA in a time-
dependent manner. This effect was due to an increased UCP gene transcription rate and
UCP mRNA stabilization, resulting in a higher content of immunoreactive mitochondrial UCP …