The synthesis of glycoconjugates within the secretory pathway of eukaryotes requires the
provision of lumenal nucleotide-sugar substrates. This is particularly important for eukaryotic
microbes such as Leishmania because they must synthesize considerable amounts of
extracellular and cell surface glycoconjugates that play significant roles in the infectious
cycle. Here we used properly oriented sealed microsomes to characterize lumenal uptake of
GDP-Man in Leishmania donovani In this system, GDP-Man uptake was saturable with an …