[HTML][HTML] Identification of a new component of the agonist binding site of the nicotinic α7 homooligomeric receptor

PJ Corringer, JL Galzi, JL Eiselé, S Bertrand… - Journal of Biological …, 1995 - ASBMB
Tryptophan 54 of the α7 neuronal nicotinic homooligomeric receptor is homologous to γ-Trp-
55 and δ-Trp-57 of non-α subunits of Torpedo receptor labeled by d-tubocurarine. This
residue was mutated on the α7-V201-5-hydroxytryptamine (5HT) 3 homooligomeric chimera,
which displays α7 nicotinic pharmacology, and for which both equilibrium binding studies
and electrophysiological recordings could be carried out in parallel. Replacement of Trp-54
by a Phe, Ala, or His causes a progressive decrease both in binding affinity and in …