Fluorimetric determination of oxidised and reduced glutathione in cells and tissues by high-performance liquid chromatography following derivatization with dansyl …
J Martin, INH White - Journal of Chromatography B: Biomedical Sciences …, 1991 - Elsevier
A high-performance liquid chromatographic method utilising fluorimetric detection of
oxidised and reduced glutathione, following derivatization with dansyl chloride is described.
Dansyl derivatives are separated on an aminopropyl silica column with a methanol—sodium
acetate gradient system giving detection limits (signal-to-noise ratio= 2) of 1 pmol. This is in
the order of 100-fold more sensitive than established methods based on the ultraviolet
detection of dinitrophenylglutathione derivatives. The present procedures have been used to …
oxidised and reduced glutathione, following derivatization with dansyl chloride is described.
Dansyl derivatives are separated on an aminopropyl silica column with a methanol—sodium
acetate gradient system giving detection limits (signal-to-noise ratio= 2) of 1 pmol. This is in
the order of 100-fold more sensitive than established methods based on the ultraviolet
detection of dinitrophenylglutathione derivatives. The present procedures have been used to …