[HTML][HTML] Synthesis of prostaglandin E2 ethanolamide from anandamide by cyclooxygenase-2
M Yu, D Ives, CS Ramesha - Journal of Biological Chemistry, 1997 - ASBMB
Because of its structural similarity to polyunsaturated fatty acids, anandamide could serve as
substrate for enzymes such as lipoxygenases and cyclooxygenases, which metabolize
polyunsaturated fatty acids to potent bioactive metabolites. Here the ability of recombinant
human cyclooxygenase-1 (hCOX-1) and cyclooxygenase-2 (hCOX-2) to metabolize
anandamide was studied. Baculovirus-expressed and-purified hCOX-2, but not hCOX-1,
effectively oxygenated anandamide. Reverse phase high pressure liquid chromatography …
substrate for enzymes such as lipoxygenases and cyclooxygenases, which metabolize
polyunsaturated fatty acids to potent bioactive metabolites. Here the ability of recombinant
human cyclooxygenase-1 (hCOX-1) and cyclooxygenase-2 (hCOX-2) to metabolize
anandamide was studied. Baculovirus-expressed and-purified hCOX-2, but not hCOX-1,
effectively oxygenated anandamide. Reverse phase high pressure liquid chromatography …