Novel fatty acid beta-oxidation enzymes in rat liver mitochondria. II. Purification and properties of enoyl-coenzyme A (CoA) hydratase/3-hydroxyacyl-CoA …
Y Uchida, K Izai, T Orii, T Hashimoto - Journal of Biological Chemistry, 1992 - ASBMB
Long-chain 3-hydroxyacyl-CoA dehydrogenase was extracted from the washed membrane
fraction of frozen rat liver mitochondria with buffer containing detergent and then was
purified. This enzyme is an oligomer with a molecular mass of 460 kDa and consisted of 4
mol of large polypeptide (79 kDa) and 4 mol of small polypeptides (51 and 49 kDa). The
purified enzyme preparation was concluded to be free from the following enzymes based on
marked differences in behavior of the enzyme during purification, molecular masses of the …
fraction of frozen rat liver mitochondria with buffer containing detergent and then was
purified. This enzyme is an oligomer with a molecular mass of 460 kDa and consisted of 4
mol of large polypeptide (79 kDa) and 4 mol of small polypeptides (51 and 49 kDa). The
purified enzyme preparation was concluded to be free from the following enzymes based on
marked differences in behavior of the enzyme during purification, molecular masses of the …