Transformation of arachidonic acid and homo-gamma-linolenic acid by rabbit polymorphonuclear leukocytes. Monohydroxy acids from novel lipoxygenases
P Borgeat, M Hamberg, B Samuelsson - Journal of Biological Chemistry, 1976 - ASBMB
Addition of arachidonic acid and homo-gamma-linolenic acid to a suspension of rabbit
peritoneal neutrophils led to the synthesis of 5-L-hydroxy-6, 8, 11, 14-eicosatetraenoic acid
and 8-L-hydroxy-9, 11, 14-eicosatrienoic acid, respectively. Both hydroxy acids were found
to be the main metabolites of their respective unsaturated C-20 fatty acid precursor,
constituting more than 50% of the total substrate conversion. The formation of the two
metabolites was not inhibted bb indomethacin, indicating that the enzymes involved were …
peritoneal neutrophils led to the synthesis of 5-L-hydroxy-6, 8, 11, 14-eicosatetraenoic acid
and 8-L-hydroxy-9, 11, 14-eicosatrienoic acid, respectively. Both hydroxy acids were found
to be the main metabolites of their respective unsaturated C-20 fatty acid precursor,
constituting more than 50% of the total substrate conversion. The formation of the two
metabolites was not inhibted bb indomethacin, indicating that the enzymes involved were …