[HTML][HTML] Essential Requirement of Cytosolic Phospholipase A2for Activation of the Phagocyte NADPH Oxidase
R Dana, TL Leto, HL Malech, R Levy - Journal of Biological Chemistry, 1998 - ASBMB
Arachidonic acid (AA) can trigger activation of the phagocyte NADPH oxidase in a cell-free
assay. However, a role for AA in activation of the oxidase in intact cells has not been
established, nor has the AA generating enzyme critical to this process been identified. The
human myeloid cell line PLB-985 was transfected to express p85 cytosolic phospholipase A
2 (cPLA 2) antisense mRNA and stable clones were selected that lack detectable cPLA 2.
cPLA 2-deficient PLB-985 cells differentiate similarly to control PLB-985 cells in response to …
assay. However, a role for AA in activation of the oxidase in intact cells has not been
established, nor has the AA generating enzyme critical to this process been identified. The
human myeloid cell line PLB-985 was transfected to express p85 cytosolic phospholipase A
2 (cPLA 2) antisense mRNA and stable clones were selected that lack detectable cPLA 2.
cPLA 2-deficient PLB-985 cells differentiate similarly to control PLB-985 cells in response to …