Lipoxygenase metabolism of endogenous and exogenous arachidonate in leukocytes: GC-MS analyses of incubations in H218O buffers
AR Brash, CD Ingram - Prostaglandins, Leukotrienes and Medicine, 1986 - Elsevier
Fatty acids are labeled with 18 O in the carboxyl group during ester hydrolysis in H 2 18 O.
We utilized this principle to develop a novel mass spectrometric method for study of the
turnover of arachidonic acid in intact cell systems. Analysis of 18 O incorporations was by
negative ion chemical ionization GC-MS. The use of deuterium-labeled exogenous
substrates allowed the metabolic fate of exogenous and intrinsic compounds to be
distinguished. Thus, it was shown that the preferred route of 5-lipoxygenase metabolism of …
We utilized this principle to develop a novel mass spectrometric method for study of the
turnover of arachidonic acid in intact cell systems. Analysis of 18 O incorporations was by
negative ion chemical ionization GC-MS. The use of deuterium-labeled exogenous
substrates allowed the metabolic fate of exogenous and intrinsic compounds to be
distinguished. Thus, it was shown that the preferred route of 5-lipoxygenase metabolism of …