Homocysteine-induced endothelial cell injury in vitro: a model for the study of vascular injury
RT Wall, JM Harlan, LA Harker, GE Striker - Thrombosis research, 1980 - Elsevier
Direct chemical injury to vascular endothelium was determined in vitro by measuring
independently cell detachment and release of 51 Cr from labeled human endothelial cell
monolayers. Homocysteine, a sulfhydryl amino acid, induced specific 51 Cr release from
endothelial cells in direct proportion to its concentration between 0.1 and 10 mM. The
proportion of detached cells during exposure to homocysteine was also directly related to
the concentration of homocysteine. In vitro preincubation of homocysteine resulted in a …
independently cell detachment and release of 51 Cr from labeled human endothelial cell
monolayers. Homocysteine, a sulfhydryl amino acid, induced specific 51 Cr release from
endothelial cells in direct proportion to its concentration between 0.1 and 10 mM. The
proportion of detached cells during exposure to homocysteine was also directly related to
the concentration of homocysteine. In vitro preincubation of homocysteine resulted in a …