Effects of insulin on steady state kinetics of GLUT4 subcellular distribution in rat adipocytes. Evidence of constitutive GLUT4 recycling.
BH Jhun, AL Rampal, H Liu, M Lachaal… - Journal of Biological …, 1992 - ASBMB
We labeled rat adipocyte cell surface glucose transporters with an impermeable,
photoreactive glucose analogue, 1, 3-bis-(3-deoxy-D-glucopyranose-3-yloxy)-2-propyl 4-
benzoylbenzoate (B3GL) and its radioactive tracer [3H] B3GL. The labeling did not affect
glucose transporter subcellular distribution in basal and insulin-stimulated adipocytes. When
basal or insulin-stimulated adipocytes were labeled with [3H] B3GL and incubated at 37
degrees C in steady state, labeled GLUT4 was rapidly reduced at the cell surface and …
photoreactive glucose analogue, 1, 3-bis-(3-deoxy-D-glucopyranose-3-yloxy)-2-propyl 4-
benzoylbenzoate (B3GL) and its radioactive tracer [3H] B3GL. The labeling did not affect
glucose transporter subcellular distribution in basal and insulin-stimulated adipocytes. When
basal or insulin-stimulated adipocytes were labeled with [3H] B3GL and incubated at 37
degrees C in steady state, labeled GLUT4 was rapidly reduced at the cell surface and …