Phenotypic and functional characterization in vitro of a multipotent epithelial cell present in the normal adult human breast
J Stingl, JT Emerman, CJ Eaves, U Kuusk - Differentiation, 1998 - Elsevier
The developmental relationships between the different mammary epithelial cell lineages in
the human mammary gland are not well defined. To characterize human breast epithelial
cells (HBEC) with progenitor activity, we used flow cytometry and single cell sorting to
analyze the distribution of cellular phenotypes in primary cultures of reduction
mammoplasties and their associated ability to generate colonies in 2-dimensional (D) and 3-
D (collagen gel) culture systems. This approach allowed two distinct types of HBEC …
the human mammary gland are not well defined. To characterize human breast epithelial
cells (HBEC) with progenitor activity, we used flow cytometry and single cell sorting to
analyze the distribution of cellular phenotypes in primary cultures of reduction
mammoplasties and their associated ability to generate colonies in 2-dimensional (D) and 3-
D (collagen gel) culture systems. This approach allowed two distinct types of HBEC …