The effect of proteoglycans on the morphology of collagen fibrils formed in vitro
KG Vogel, JA Trotter - Collagen and related research, 1987 - Elsevier
The morphology of collagen fibrils at various times during formation in vitro was
quantitatively examined by negative staining and by scanning electron microscopy. The
presence of a small dermatan sulfate proteoglycan from bovine tendon (5 μg
proteoglycan/100 μg collagen) resulted in collagen fibrils that were significantly thinner in
width at all times by both methodologies. The rate of fibril diameter increase was also
retarded by the small proteoglycans, suggesting that they inhibited the lateral aggregation of …
quantitatively examined by negative staining and by scanning electron microscopy. The
presence of a small dermatan sulfate proteoglycan from bovine tendon (5 μg
proteoglycan/100 μg collagen) resulted in collagen fibrils that were significantly thinner in
width at all times by both methodologies. The rate of fibril diameter increase was also
retarded by the small proteoglycans, suggesting that they inhibited the lateral aggregation of …