Objectives To determine the role of extracellular proteinases in ischemia-induced retinal
neovascularization in an animal model and to examine the effect of proteinase inhibitors on
retinal neovascularization. Methods Retinal neovascularization was induced in newborn
mice exposed to 75% oxygen for 5 days, followed by room air. Retinal extracts underwent
zymographic analysis to measure the activity of urokinase and matrix metalloproteinases
(MMPs). Some animals under the same conditions also received intraperitoneal injections of …