The human thymic dendritic cell phenotype and its modification in culture
M Lafontaine, D Landry, S Montplaisir - Cellular immunology, 1992 - Elsevier
In order to extend our study of human thymic dendritic cells (DC) we have purified DC by
density gradient separation followed by treatment with CD1 and CD2 m Ab and antibody-
coated immunobeads. The resulting population contains 60 to 75% brightly HLA-DR+ cells.
Morphological and functional studies demonstrate that these cells share the common
characteristics of dendritic cells. Extensive phenotypic analysis of the purified DC has been
made using a panel of mAb. Cytofluorometric assays with mAb reactive with common …
density gradient separation followed by treatment with CD1 and CD2 m Ab and antibody-
coated immunobeads. The resulting population contains 60 to 75% brightly HLA-DR+ cells.
Morphological and functional studies demonstrate that these cells share the common
characteristics of dendritic cells. Extensive phenotypic analysis of the purified DC has been
made using a panel of mAb. Cytofluorometric assays with mAb reactive with common …