Concordant induction of prostaglandin E2synthase with cyclooxygenase-2 leads to preferred production of prostaglandin E2over thromboxane and prostaglandin …
H Matsumoto, H Naraba, M Murakami, I Kudo… - Biochemical and …, 1997 - Elsevier
H Matsumoto, H Naraba, M Murakami, I Kudo, K Yamaki, A Ueno, S Oh-ishi
Biochemical and biophysical research communications, 1997•ElsevierRat peritoneal macrophages were stimulated with lipopolysaccaride (LPS) for various
periods and their ability to convert exogenous arachidonic acid to various prostanoids was
examined. Unstimulated cells, which expressed cyclooxygenase (COX)-1 but not COX-2,
produced thromboxane (TX) B2> prostaglandin (PG) D2> PGE2, whereas cells stimulated
for 6–12 h with LPS exhibited marked increase in conversion to PGE2, which paralleled
COX-2 induction, with minimal change in conversion to TXB2and PGD2. Pharmacological …
periods and their ability to convert exogenous arachidonic acid to various prostanoids was
examined. Unstimulated cells, which expressed cyclooxygenase (COX)-1 but not COX-2,
produced thromboxane (TX) B2> prostaglandin (PG) D2> PGE2, whereas cells stimulated
for 6–12 h with LPS exhibited marked increase in conversion to PGE2, which paralleled
COX-2 induction, with minimal change in conversion to TXB2and PGD2. Pharmacological …
Rat peritoneal macrophages were stimulated with lipopolysaccaride (LPS) for various periods and their ability to convert exogenous arachidonic acid to various prostanoids was examined. Unstimulated cells, which expressed cyclooxygenase (COX)-1 but not COX-2, produced thromboxane (TX) B2> prostaglandin (PG) D2> PGE2, whereas cells stimulated for 6–12 h with LPS exhibited marked increase in conversion to PGE2, which paralleled COX-2 induction, with minimal change in conversion to TXB2and PGD2. Pharmacological studies showed that formation of PGE2was mediated predominantly by COX-2, PGD2by COX-1, and TXB2by both COX-1 and COX-2 depending upon the timing of LPS stimulation. Measurement of the conversion of exogenous PGH2to each prostanoid in cell lysates demonstrated LPS-dependent increase in PGE2synthase activity that was degenerated by pretreatment with actinomycin D or cycloheximide. Thus, concordant induction of terminal PGE2synthase with COX-2 leads to the preferred production of PGE2to TXB2and PGD2by LPS-stimulated macrophages.
Elsevier