Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes
SL Mansour, KR Thomas, MR Capecchi - Nature, 1988 - nature.com
SL Mansour, KR Thomas, MR Capecchi
Nature, 1988•nature.comGene targeting—homologous recombination of DNA sequences residing in the
chromosome with newly introduced DNA sequences—in mouse embryo-derived stem cells
promises to provide a means to generate mice of any desired genotype. We describe a
positive and negative selection procedure that enriches 2,000-fold for those cells that
contain a targeted mutation. The procedure was applied to the isolation of hprt− and int-2−
mutants, but it should be applicable to any gene
chromosome with newly introduced DNA sequences—in mouse embryo-derived stem cells
promises to provide a means to generate mice of any desired genotype. We describe a
positive and negative selection procedure that enriches 2,000-fold for those cells that
contain a targeted mutation. The procedure was applied to the isolation of hprt− and int-2−
mutants, but it should be applicable to any gene
Abstract
Gene targeting—homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences—in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive and negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt− and int-2− mutants, but it should be applicable to any gene
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