Aspergillus fumigatus antigen induced eosinophilia in mice is abrogated by anti-IL-5 antibody

PS Murali, A Kumar, H Choi, NK Bansal… - Journal of leukocyte …, 1993 - academic.oup.com
PS Murali, A Kumar, H Choi, NK Bansal, JN Fink, VP Kurup
Journal of leukocyte biology, 1993academic.oup.com
A murine model of allergic bronchopulmonary aspergillosis (ABPA), developed by exposure
to Aspergillus fumigatus antigens, demonstrated eosinophilia of peripheral blood (PB), bone
marrow (BM), and lung. The eosinophilia was abrogated by monoclonal anti-inter-leukin-5
(IL-5) antibody (TRFK-5) and not by an isotype control antibody (GL 113). Eosinophils in PB
were enumerated from stained smears and their relative increase or decrease in cells from
BM and lung was determined by an eosinophil peroxidase (EPO) assay (measured in …
Abstract
A murine model of allergic bronchopulmonary aspergillosis (ABPA), developed by exposure to Aspergillus fumigatus antigens, demonstrated eosinophilia of peripheral blood (PB), bone marrow (BM), and lung. The eosinophilia was abrogated by monoclonal anti-inter-leukin-5 (IL-5) antibody (TRFK-5) and not by an isotype control antibody (GL 113). Eosinophils in PB were enumerated from stained smears and their relative increase or decrease in cells from BM and lung was determined by an eosinophil peroxidase (EPO) assay (measured in optical density). Intraperitoneal injection of TRFK-5 in mice exposed to A. fumigatus antigen produced a significant reduction in eosinophils (PB 6.6 ± 1.14% vs. 3.8 ± 0.8%, P< .01) and EPO production in BM (0.935 ± 0.03 vs. 0.615 ± 0.02, P < .001). A similar reduction in EPO production in the lung (0.691 ± 0.12 vs. 0.495 ± 0.05, not significant) was also reflected in the histopathol-ogy for the different groups of mice. These findings confirming the role of IL-5 in eosinophilia, although not surprising, are significant in elucidating the immu-nopathogenesis of ABPA in the murine model. We conclude that in this model, eosinophilia may be due largely to the Th2 cytokine -IL-5 induced by A. fumigatus antigens. J. Leukoc. Biol. 53: 264–267; 1993.
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