A novel method of purifying lung surfactant proteins A and D from the lung lavage of alveolar proteinosis patients and from pooled amniotic fluid
P Strong, U Kishore, C Morgan, AL Bernal… - Journal of …, 1998 - Elsevier
A simple procedure has been developed for the purification of the surfactant proteins SP-A
and SP-D from lung lavage of patients with alveolar proteinosis. The SP-D is purified by
fractionation of the supernatant obtained after spinning the lavage at 10 000× g for 40 min,
while the bulk of the SP-A is purified by fractionation of the pellet. The supernatant is applied
to a maltosyl–agarose column and the bound SP-D is specifically eluted using MnCl2. The
pellet is solubilised in 6 M urea and, following renaturation, the solubilised proteins are …
and SP-D from lung lavage of patients with alveolar proteinosis. The SP-D is purified by
fractionation of the supernatant obtained after spinning the lavage at 10 000× g for 40 min,
while the bulk of the SP-A is purified by fractionation of the pellet. The supernatant is applied
to a maltosyl–agarose column and the bound SP-D is specifically eluted using MnCl2. The
pellet is solubilised in 6 M urea and, following renaturation, the solubilised proteins are …