The effect of nociceptin on Ca2+ channel current and intracellular Ca2+ in the SH‐SY5Y human neuroblastoma cell line
M Connor, A Yoe, G Henderson - British journal of …, 1996 - Wiley Online Library
M Connor, A Yoe, G Henderson
British journal of pharmacology, 1996•Wiley Online LibraryThe human neuroblastoma cell line SH‐SY5Y expresses the 'orphan'opioid receptor
(ORL1). We have demonstrated that nociceptin, the putative endogenous ligand for ORL1,
produces a concentration‐dependent inhibition of the N‐type calcium channel current in
these cells (IC50 42 nM). In addition, in the presence of carbachol, nociceptin increased the
intracellular concentration of Ca2+ (EC50 60 nM). Both effects of nociceptin were blocked by
pertussis toxin pretreatment but not by the opioid antagonists CTAP (1 μm), naltrindole (1 …
(ORL1). We have demonstrated that nociceptin, the putative endogenous ligand for ORL1,
produces a concentration‐dependent inhibition of the N‐type calcium channel current in
these cells (IC50 42 nM). In addition, in the presence of carbachol, nociceptin increased the
intracellular concentration of Ca2+ (EC50 60 nM). Both effects of nociceptin were blocked by
pertussis toxin pretreatment but not by the opioid antagonists CTAP (1 μm), naltrindole (1 …
The human neuroblastoma cell line SH‐SY5Y expresses the ‘orphan’ opioid receptor (ORL1). We have demonstrated that nociceptin, the putative endogenous ligand for ORL1, produces a concentration‐dependent inhibition of the N‐type calcium channel current in these cells (IC50 42 nM). In addition, in the presence of carbachol, nociceptin increased the intracellular concentration of Ca2+ (EC50 60 nM). Both effects of nociceptin were blocked by pertussis toxin pretreatment but not by the opioid antagonists CTAP (1 μm), naltrindole (1 μm) and naloxone (10 μm).
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