Regulation of CS1 fibronectin expression and function by IL-1 in endothelial cells

DL Boyle, Y Shi, S Gay, GS Firestein - Cellular immunology, 2000 - Elsevier
VLA-4 is a critical adhesion molecule that regulates mononuclear cell trafficking to sites of
inflammation. VCAM-1 is a primary ligand of VLA-4, although alternatively spliced fibronectin
(FN) containing the CS1 region (CS1 FN) also binds to VLA-4. CS1 FN is expressed by
rheumatoid arthritis (RA) synovial endothelial cells, but the factors that regulate CS1 FN
expression are not known. We incubated human umbilical vein endothelial cells (HUVEC)
with IL-1 (0.1–10 ng/ml) for 8–48 h and determined total FN and CS1 FN mRNA by Northern …