Radioimmunoassays for glutamic acid decarboxylase (GAD65) and GAD65 autoantibodies using 35S or 3H recombinant human ligands
A Falorni, E Örtqvist, B Persson, Å Lernmark - Journal of immunological …, 1995 - Elsevier
Autoantibodies are an important marker of human autoimmune diseases and the
development of simple, precise and reproducible immunoassays to detect autoantibodies is
important to our understanding of human autoimmunity. GAD65 autoantibodies occur
frequently in insulin-dependent diabetic patients and is a useful marker for IDDM. A RIA to
detect immunoreactive GAD65 has not been described. In the present study we describe a
semi-automated fluid-phase immunoassay for the rapid detection of GAD65 autoantibodies …
development of simple, precise and reproducible immunoassays to detect autoantibodies is
important to our understanding of human autoimmunity. GAD65 autoantibodies occur
frequently in insulin-dependent diabetic patients and is a useful marker for IDDM. A RIA to
detect immunoreactive GAD65 has not been described. In the present study we describe a
semi-automated fluid-phase immunoassay for the rapid detection of GAD65 autoantibodies …