[CITATION][C] Assay and purification of naturally occurring inhibitor of angiogenesis

PJ Polverini, NP Bouck, F Rastinejad - Methods in enzymology, 1991 - Elsevier
A wide variety of tissues, cells, and cell extracts or media conditioned by cells grown in
culture have been examined for angiogenic stimulatory or inhibitory activity in the cornea.
When possible, cell extracts or conditioned media are the preferred form of material to be
assayed. One can more accurately determine the dosage/potency of a particular test sample
and precisely control the quantity and positioning of materials within the corneal stroma,
after incorporation into one of several slow-release noninflammatory polymers. Since the …