The structure and physicochemical properties of rat liver macrophage migration inhibitory factor

J Nishihira, T Kuriyama, M Sakai, S Nishi, S Ohki… - … et Biophysica Acta (BBA …, 1995 - Elsevier
We expressed rat macrophage migration inhibitory factor (rMIF) in E. coli using the cDNA
isolated from a rat liver cDNA library. rMIF specifically bound glutathione (dissociation
constant= 500 μM). We purified rMIF homogeneously on SDS-PAGE by S-hexylglutathione
Sepharose affinity column chromatography and Sephadex G-100 column chromatography.
The amino-acid sequence of rMIF was highly homologous to that of human MIF from a T-cell
line; only a single amino-acid residue was substituted if conservative amino-acid …