Buoyant density of hepatitis C virus recovered from infected hosts: two different features in sucrose equilibrium density-gradient centrifugation related to degree of liver …

T Kanto, N Hayashi, T Takehara, H Hagiwara, E Mita… - Hepatology, 1994 - journals.lww.com
Hepatitis C virus is reported to have a low buoyant density in sucrose. To determine the
density of hepatitis C virus in the circulation of infected hosts and its association with the
degree of liver inflammation, we examined serum samples from 10 patients who were
positive for both hepatitis C virus antibody (C100 antigen) antibody and serum hepatitis C
virus RNA. After the serum was ultracentrifuged in sucrose density gradient (10 to 60), the
hepatitis C virus RNA titer in each collected fraction was quantified by means of competitive …