[HTML][HTML] Chemical Modification of Hammerhead Ribozymes: CATALYTIC ACTIVITY AND NUCLEASE RESISTANCE (∗)
L Beigelman, JA McSwiggen, KG Draper… - Journal of biological …, 1995 - ASBMB
A systematic study of selectively modified, 36-mer hammerhead ribozymes has resulted in
the identification of a generic, catalytically active and nuclease stable ribozyme motif
containing 5 ribose residues, 29-30 2′-O-Me nucleotides, 1-2 other 2′-modified
nucleotides at positions U4 and U7, and a 3′-3′-linked nucleotide" cap." Eight 2′-
modified uridine residues were introduced at positions U4 and/or U7. From the resulting set
of ribozymes, several have almost wild-type catalytic activity and significantly improved …
the identification of a generic, catalytically active and nuclease stable ribozyme motif
containing 5 ribose residues, 29-30 2′-O-Me nucleotides, 1-2 other 2′-modified
nucleotides at positions U4 and U7, and a 3′-3′-linked nucleotide" cap." Eight 2′-
modified uridine residues were introduced at positions U4 and/or U7. From the resulting set
of ribozymes, several have almost wild-type catalytic activity and significantly improved …