[HTML][HTML] Purification of a 3β-hydroxy-Δ5-C27-steroid dehydrogenase from pig liver microsomes active in major and alternative pathways of bile acid biosynthesis

C Furster, J Zhang, A Toll - Journal of Biological Chemistry, 1996 - ASBMB
A 3β-hydroxy-Δ 5-C 27-steroid dehydrogenase active in bile acid biosynthesis was purified
from pig liver microsomes by solubilization with sodium cholate and by chromatography on
DEAE-Sepharose, aminohexyl-Sepharose, and blue Sepharose. The last step in the
purification procedure was preparative isoelectric focusing in a Rotofor cell. The final
enzyme preparation showed only one protein band upon SDS-polyacrylamide gel
electrophoresis. The isoelectric point was estimated to about 7.0 and the apparent M r was …