[HTML][HTML] cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism
EG Lund, TA Kerr, J Sakai, WP Li… - Journal of Biological …, 1998 - ASBMB
Oxysterols regulate the expression of genes involved in cholesterol and lipid metabolism
and serve as intermediates in cholesterol catabolism. Among the most potent of regulatory
oxysterols is 25-hydroxycholesterol, whose biosynthetic enzyme has not yet been isolated.
Here, we report the cloning of cholesterol 25-hydroxylase cDNAs from the mouse and
human. The encoded enzymes are polytopic membrane proteins of 298 and 272 amino
acids, respectively, which contain clusters of histidine residues that are essential for catalytic …
and serve as intermediates in cholesterol catabolism. Among the most potent of regulatory
oxysterols is 25-hydroxycholesterol, whose biosynthetic enzyme has not yet been isolated.
Here, we report the cloning of cholesterol 25-hydroxylase cDNAs from the mouse and
human. The encoded enzymes are polytopic membrane proteins of 298 and 272 amino
acids, respectively, which contain clusters of histidine residues that are essential for catalytic …