[HTML][HTML] Tumor necrosis factor-α-inducible IκBα proteolysis mediated by cytosolic m-calpain: a mechanism parallel to the ubiquitin-proteasome pathway for nuclear …
The cytokine tumor necrosis factor α (TNF-α) induces expression of inflammatory gene
networks by activating cytoplasmic to nuclear translocation of the nuclear factor-κB (NF-κB)
transcription factor. NF-κB activation results from sequential phosphorylation and hydrolysis
of the cytoplasmic inhibitor, IκBα, through the 26 S proteasome. Here, we show a parallel
proteasome-independent pathway for cytokine-inducible IκBα proteolysis in HepG2 liver
cells mediated by cytosolic calcium-activated neutral protease (calpains). Pretreatment with …
networks by activating cytoplasmic to nuclear translocation of the nuclear factor-κB (NF-κB)
transcription factor. NF-κB activation results from sequential phosphorylation and hydrolysis
of the cytoplasmic inhibitor, IκBα, through the 26 S proteasome. Here, we show a parallel
proteasome-independent pathway for cytokine-inducible IκBα proteolysis in HepG2 liver
cells mediated by cytosolic calcium-activated neutral protease (calpains). Pretreatment with …