Cold preservation of kidneys is commonly used in human transplantation and in vitro
studies. However, although disruption of the cytoskeleton by cold has been demonstrated in
cultured cells, the effect of cold treatment on intact kidney is poorly understood. In this study,
specific antibodies were used to examine the effect of hypothermia on the cytoskeletal
network and the trafficking of some membrane proteins in the urinary tubule. Rat kidneys
were cut into thin slices (approximately 0.5 mm) that were divided into several groups:(1) …