[HTML][HTML] Regulation of clusterin gene expression by transforming growth factor β
Transforming growth factor β (TGFβ) induces the expression of a wide variety of genes in
many cell types. Our previous studies have shown that TGFβ stimulates both clusterin mRNA
and protein levels, and induces its accumulation in the nucleus of CCL64 cells. To further
investigate the molecular mechanism of clusterin mRNA induction by TGFβ, we created a
1.3-kilobase rat clusterin promoter/luciferase reporter construct. We demonstrate that TGFβ
enhances luciferase activity 2.5–6-fold in transient transfection assays of epithelial …
many cell types. Our previous studies have shown that TGFβ stimulates both clusterin mRNA
and protein levels, and induces its accumulation in the nucleus of CCL64 cells. To further
investigate the molecular mechanism of clusterin mRNA induction by TGFβ, we created a
1.3-kilobase rat clusterin promoter/luciferase reporter construct. We demonstrate that TGFβ
enhances luciferase activity 2.5–6-fold in transient transfection assays of epithelial …