Isozyme-specific inhibition of protein kinase C by RNA aptamers.
R Conrad, LM Keranen, AD Ellington… - Journal of Biological …, 1994 - ASBMB
In vitro selection technology has been used to purify RNA aptamers from a random
sequence pool that can bind to, and specifically inhibit, protein kinase C beta II. Two of the
selected RNA aptamers bind to this isozyme of protein kinase C with nanomolar affinities
and inhibit activation with unprecedented selectivity; the highly related, alternatively spliced
beta I isozyme, which differs by 23 residues, is inhibited with 1 order of magnitude lower
potency; the next most similar isozyme, alpha, shows no detectable inhibition. The …
sequence pool that can bind to, and specifically inhibit, protein kinase C beta II. Two of the
selected RNA aptamers bind to this isozyme of protein kinase C with nanomolar affinities
and inhibit activation with unprecedented selectivity; the highly related, alternatively spliced
beta I isozyme, which differs by 23 residues, is inhibited with 1 order of magnitude lower
potency; the next most similar isozyme, alpha, shows no detectable inhibition. The …