Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
P Chomczynski, N Sacchi - Analytical biochemistry, 1987 - Elsevier
Analytical biochemistry, 1987•Elsevier
A new method of total RNA isolation by a single extraction with an acid guanidinium
thiocyanate-phenol-chloroform mixture is described. The method provides a pure
preparation of undegraded RNA in high yield and can be completed within 4 h. It is
particularly useful for processing large numbers of samples and for isolation of RNA from
minute quantities of cells or tissue samples.
thiocyanate-phenol-chloroform mixture is described. The method provides a pure
preparation of undegraded RNA in high yield and can be completed within 4 h. It is
particularly useful for processing large numbers of samples and for isolation of RNA from
minute quantities of cells or tissue samples.
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
Elsevier