[HTML][HTML] Single amino acids determine specificity of binding of protein kinase A regulatory subunits by protein kinase A anchoring proteins
K Miki, EM Eddy - Journal of Biological Chemistry, 1999 - ASBMB
Cyclic AMP-dependent protein kinase is tethered to protein kinase A anchoring proteins
(AKAPs) through regulatory subunits (R) by RIα-specific, RIIα-specific, or RIα/RIIα dual-
specific binding. Ala-and Val-scanning mutagenesis determined that hydrophobic amino
acids at three homologous positions are required for binding of RIα to FSC1/AKAP82
domain B and RIIα to AKAP Ht31. A mutation at the middle position reversed the binding
specificity of both AKAPs, and mutations at this same position of the dual-specific domain A …
(AKAPs) through regulatory subunits (R) by RIα-specific, RIIα-specific, or RIα/RIIα dual-
specific binding. Ala-and Val-scanning mutagenesis determined that hydrophobic amino
acids at three homologous positions are required for binding of RIα to FSC1/AKAP82
domain B and RIIα to AKAP Ht31. A mutation at the middle position reversed the binding
specificity of both AKAPs, and mutations at this same position of the dual-specific domain A …