All-trans retinoic acid induces cellular retinol-binding protein in human skin in vivo

GJ Fisher, AP Reddy, SC Datta, S Kang… - Journal of investigative …, 1995 - Elsevier
GJ Fisher, AP Reddy, SC Datta, S Kang, YY Jong, P Chambon, JJ Voorhees
Journal of investigative dermatology, 1995Elsevier
We examined the regulation of cellular retinol-binding protein (CRBP) mRNA and protein
expression in human skin in vivo by all-trans retinoic acid and all-trans retinol. Treatment of
human skin for 24 h with all-trans retinoic acid (0.1%) or all-trans retinol (1.6%) induced
CRBP mRNA 5.5-fold (p< 0.01, n= 10) and 5.7-fold (p< 0.01, n= 5), respectively, compared
with skin treated with vehicle or sodium lauryl sulfate (used as an irritant control). In vitro
translation of poly A+ RNA from all-trans retinoic acid, all-trans retinol, sodium lauryl sulfate …
We examined the regulation of cellular retinol-binding protein (CRBP) mRNA and protein expression in human skin in vivo by all-trans retinoic acid and all-trans retinol. Treatment of human skin for 24 h with all-trans retinoic acid (0.1%) or all-trans retinol (1.6%) induced CRBP mRNA 5.5-fold (p < 0.01, n = 10) and 5.7-fold (p < 0.01, n = 5), respectively, compared with skin treated with vehicle or sodium lauryl sulfate (used as an irritant control). In vitro translation of poly A+ RNA from all-trans retinoic acid, all-trans retinol, sodium lauryl sulfate, and vehicle-treated human skin demonstrated that the observed increased CRBP mRNA in all-trans retinoic acid- and all-trans retinol-treated skin was able to direct increased (2.3-2.9-fold) CRBP protein synthesis. Riboprobe in situ hybridization revealed that CRBP mRNA was uniformly elevated throughout the epidermis and in dermal cells after all-trans retinoic acid treatment of human skin. Western analysis revealed that CRBP protein was elevated 3.2-fold (p 0.01, n = 6) and 3.0-fold (p < 0.01, n = 6) after all-trans retinoic acid treatment of human skin in vivo for 24 and 96 h, respectively, compared with vehicle and sodium lauryl sulfate-treated skin. In addition, functional CRBP levels measured by [3H]all-trans retinol binding were elevated 1.9-fold (p < 0.01, n = 6) and 3.5-fold (p < 0.01, n = 6) at 24 and 94 h, respectively, after all-trans retinoic acid treatment, compared with vehicle- or sodium lauryl sulfatetreated skin. Gel mobility shift analysis revealed that retinoid receptors in nuclear extracts from human skin formed a specific complex with a DNA probe containing the retinoic acid response element in the mouse CRBP gene. Monoclonal antibodies to nuclear retinoid receptors demonstrated that predominantly retinoic acid receptor-α/retinoid X receptor-α heterodimers bound to the CRBP retinoic acid response element. These data demonstrate that CRBP expression in human skin in vivo is regulated by exogenous all-trans retinoic acid and all-trans retinol.
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