Loss of Apc+ in Intestinal Adenomas from Min Mice
C Luongo, AR Moser, S Gledhill, WF Dove - Cancer research, 1994 - AACR
C Luongo, AR Moser, S Gledhill, WF Dove
Cancer research, 1994•AACRAllelic loss at the Apc locus in spontaneously occurring intestinal adenomas from mice
heterozygous for the ApcMin nonsense mutation was analyzed using a site-specific
quantitative polymerase chain reaction assay. All 97 of the intestinal adenomas analyzed
showed extensive loss of the wild-type Apc (Apc+) allele. Quantitative polymerase chain
reaction analysis of loci linked to Apc indicated loss of the chromosome carrying Apc+. Only
one copy of the homologue carrying ApcMin remained in the intestinal adenomas. Possible …
heterozygous for the ApcMin nonsense mutation was analyzed using a site-specific
quantitative polymerase chain reaction assay. All 97 of the intestinal adenomas analyzed
showed extensive loss of the wild-type Apc (Apc+) allele. Quantitative polymerase chain
reaction analysis of loci linked to Apc indicated loss of the chromosome carrying Apc+. Only
one copy of the homologue carrying ApcMin remained in the intestinal adenomas. Possible …
Abstract
Allelic loss at the Apc locus in spontaneously occurring intestinal adenomas from mice heterozygous for the ApcMin nonsense mutation was analyzed using a site-specific quantitative polymerase chain reaction assay. All 97 of the intestinal adenomas analyzed showed extensive loss of the wild-type Apc (Apc+) allele. Quantitative polymerase chain reaction analysis of loci linked to Apc indicated loss of the chromosome carrying Apc+. Only one copy of the homologue carrying ApcMin remained in the intestinal adenomas. Possible reasons for the difference in the mechanism of Apc+ loss between human and Min mouse intestinal adenomas are discussed.
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