Determination of phospholipid oxidation in cultured cells
GT Shwaery, JM Samii, B Frei, JF Keaney Jr - Methods in enzymology, 1999 - Elsevier
Publisher Summary This chapter discusses the use of high-performance liquid
chromatography (HPLC) with postcolumn chemiluminescence detection for the
measurement of lipid peroxidation in cultured cells. In this assay, extracted phospholipids
are separated by HPLC over a silica column and individual lipid classes are detected by UV
(ultra violet) absorption. In the postcolumn chemiluminescence assay, microperoxidase
catalyzes the decomposition of hydroperoxides to the corresponding alkoxyl radicals. This …
chromatography (HPLC) with postcolumn chemiluminescence detection for the
measurement of lipid peroxidation in cultured cells. In this assay, extracted phospholipids
are separated by HPLC over a silica column and individual lipid classes are detected by UV
(ultra violet) absorption. In the postcolumn chemiluminescence assay, microperoxidase
catalyzes the decomposition of hydroperoxides to the corresponding alkoxyl radicals. This …