[25] In vitro RNA synthesis with SP6 RNA polymerase
PA Krieg, DA Melton - Methods in enzymology, 1987 - Elsevier
Publisher Summary This chapter discusses the in vitro ribonucleic acid (RNA) synthesis with
SP6 RNA polymerase. The chapter describes the use of in vitro transcription systems for the
synthesis of RNAs for use as substrates and hybridization probes. The chapter discusses the
advantages of the technique and several associated problems. The system of SP6 RNA
polymerase is used as an example throughout the chapter, but the observations are stated
to be general and can be applied to any of the phage polymerase and promoter systems …
SP6 RNA polymerase. The chapter describes the use of in vitro transcription systems for the
synthesis of RNAs for use as substrates and hybridization probes. The chapter discusses the
advantages of the technique and several associated problems. The system of SP6 RNA
polymerase is used as an example throughout the chapter, but the observations are stated
to be general and can be applied to any of the phage polymerase and promoter systems …