[HTML][HTML] Defective parathyroid hormone regulation of NHE3 activity and phosphate adaptation in cultured NHERF-1-/-renal proximal tubule cells
R Cunningham, D Steplock, F Wang, H Huang… - Journal of Biological …, 2004 - ASBMB
The present experiments using primary cultures of renal proximal tubule cells derived from
wild-type and NHERF-1 knockout animals examines the regulation of NHE3 by
phenylthiohydantoin (PTH) and the regulation of phosphate transport in response to
alterations in the media content of phosphate. Forskolin (34.8±6.2%) and PTH (29.7±1.8%)
inhibited NHE3 activity in wild-type proximal tubule cells but neither forskolin (-3.2±3.3%)
nor PTH (-16.6±8.1%) inhibited NHE3 activity in NHERF-1-/-cells. Using adenovirus …
wild-type and NHERF-1 knockout animals examines the regulation of NHE3 by
phenylthiohydantoin (PTH) and the regulation of phosphate transport in response to
alterations in the media content of phosphate. Forskolin (34.8±6.2%) and PTH (29.7±1.8%)
inhibited NHE3 activity in wild-type proximal tubule cells but neither forskolin (-3.2±3.3%)
nor PTH (-16.6±8.1%) inhibited NHE3 activity in NHERF-1-/-cells. Using adenovirus …