[HTML][HTML] G protein-coupled receptor kinase 6A phosphorylates the Na+/H+ exchanger regulatory factor via a PDZ domain-mediated interaction
RA Hall, RF Spurney, RT Premont, N Rahman… - Journal of Biological …, 1999 - ASBMB
The Na+/H+ exchanger regulatory factor (NHERF) is constitutively phosphorylated in cells,
but the site (s) of this phosphorylation and the kinase (s) responsible for it have not been
identified. We show here that the primary site of constitutive NHERF phosphorylation in
human embryonic kidney 293 (HEK-293) cells is Ser 289, and that the stoichiometry of
phosphorylation is near 1 mol/mol. NHERF contains two PDZ domains that recognize the
sequence S/TXL at the carboxyl terminus of target proteins, and thus we examined the …
but the site (s) of this phosphorylation and the kinase (s) responsible for it have not been
identified. We show here that the primary site of constitutive NHERF phosphorylation in
human embryonic kidney 293 (HEK-293) cells is Ser 289, and that the stoichiometry of
phosphorylation is near 1 mol/mol. NHERF contains two PDZ domains that recognize the
sequence S/TXL at the carboxyl terminus of target proteins, and thus we examined the …