[HTML][HTML] Distinct mechanisms mediate the initial and sustained phases of integrin-mediated activation of the Raf/MEK/mitogen-activated protein kinase cascade
AK Howe, RL Juliano - Journal of Biological Chemistry, 1998 - ASBMB
Integrin-mediated adhesion to the extracellular matrix activates the canonical mitogen-
activated protein kinase cascade, although the exact mechanism is not fully resolved. We
show that integrin-mediated activation of Raf-1, an upstream regulator of mitogen-activated
protein kinase, occurs in two phases. Efficient early activation of Raf required Raf-Ras
interaction but was not affected by protein kinase C (PKC) inhibitors, while a lower,
sustained level of activity was independent of Raf-Ras interaction but was reduced by PKC …
activated protein kinase cascade, although the exact mechanism is not fully resolved. We
show that integrin-mediated activation of Raf-1, an upstream regulator of mitogen-activated
protein kinase, occurs in two phases. Efficient early activation of Raf required Raf-Ras
interaction but was not affected by protein kinase C (PKC) inhibitors, while a lower,
sustained level of activity was independent of Raf-Ras interaction but was reduced by PKC …