[HTML][HTML] CD44 interaction with tiam1 promotes Rac1 signaling and hyaluronic acid-mediated breast tumor cell migration

LYW Bourguignon, H Zhu, L Shao, YW Chen - Journal of Biological …, 2000 - ASBMB
In this study we have explored the interaction between CD44 (the hyaluronic acid (HA)-
binding receptor) and Tiam1 (a guanine nucleotide exchange factor) in metastatic breast
tumor cells (SP1 cell line). Immunoprecipitation and immunoblot analyses indicate that both
the CD44v3 isoform and the Tiam1 protein are expressed in SP1 cells and that these two
proteins are physically associated as a complex in vivo. Using an Escherichia coli-derived
calmodulin-binding peptide-tagged Tiam1 fragment (ie the NH 2-terminal pleckstrin …