Inhibition of osteoclast proton transport by bafilomycin A1 abolishes bone resorption
K Sundquist, P Lakkakorpi, B Wallmark… - … and biophysical research …, 1990 - Elsevier
K Sundquist, P Lakkakorpi, B Wallmark, K Väänänen
Biochemical and biophysical research communications, 1990•ElsevierOsteoclasts are the main bone resorbing cells with capacity to acidify their intimate contact
area with bone. Recent studies have suggested that osteoclast acid secretion is carried out
by an H+-ATPase. We demonstrate here, that specific inhibitor of vacuolar type H+-ATPase,
bafilomycin A 1, inhibits bone resorption in osteoclast cultures as well as blocks proton
transport in isolated medullary bone derived microsomes containing a vacuolar type H+-
ATPase. These results demonstrate an important role of vacuolar H+-ATPase in bone …
area with bone. Recent studies have suggested that osteoclast acid secretion is carried out
by an H+-ATPase. We demonstrate here, that specific inhibitor of vacuolar type H+-ATPase,
bafilomycin A 1, inhibits bone resorption in osteoclast cultures as well as blocks proton
transport in isolated medullary bone derived microsomes containing a vacuolar type H+-
ATPase. These results demonstrate an important role of vacuolar H+-ATPase in bone …
Abstract
Osteoclasts are the main bone resorbing cells with capacity to acidify their intimate contact area with bone. Recent studies have suggested that osteoclast acid secretion is carried out by an H+-ATPase. We demonstrate here, that specific inhibitor of vacuolar type H+-ATPase, bafilomycin A1, inhibits bone resorption in osteoclast cultures as well as blocks proton transport in isolated medullary bone derived microsomes containing a vacuolar type H+-ATPase. These results demonstrate an important role of vacuolar H+-ATPase in bone resorption.
Elsevier