Assay of intestinal disaccharidases
A Dahlqvist - Analytical biochemistry, 1968 - Elsevier
A Dahlqvist
Analytical biochemistry, 1968•ElsevierAssay of Intestinal Disaccharidases Page 1 ANALYTICAL BIOCHEMISTRY 22, 99-107 (1968)
Assay of Intestinal Disaccharidases ARNE DAHLQVIST Research Department of the Hospital
and Department of Physiological Chemistry, University of Lund. Lund, Sweden Received May
1, 1967 A method for the assay of intestinal disaccharidase activity was described by
Dahlqvist (1). After incubation of the enzyme preparations with the appropriate substrate the
reaction was interrupted by boiling or protein precipitation, and then the glucose liberated …
Assay of Intestinal Disaccharidases ARNE DAHLQVIST Research Department of the Hospital
and Department of Physiological Chemistry, University of Lund. Lund, Sweden Received May
1, 1967 A method for the assay of intestinal disaccharidase activity was described by
Dahlqvist (1). After incubation of the enzyme preparations with the appropriate substrate the
reaction was interrupted by boiling or protein precipitation, and then the glucose liberated …
A simplified method for the assay of small intestine disaccharidase activity is described which is suitable for use both on the “standard” scale (final volume 3.2 ml) and on the “ultramicro” scale (final volume 320 μl).
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