Isolation of two distinct type I angiotensin II receptor genes
TS Elton, CC Stephan, GR Taylor, MG Kimball… - Biochemical and …, 1992 - Elsevier
TS Elton, CC Stephan, GR Taylor, MG Kimball, MM Martin, JN Durand, S Oparil
Biochemical and biophysical research communications, 1992•ElsevierA rat genomic Southern blot, probed with a type I angiotensin II receptor probe,
demonstrated that two highly homologous type I angiotensin II receptors were present. A rat
genomic library was subsequently screened and four clones were isolated. From restriction
mapping, differential hybridization, polymerase chain reaction amplification and sequence
analyses we have determined that there are two unique type I angiotensin II receptor genes.
The first of these genes corresponds to the published rat vascular complementary DNA …
demonstrated that two highly homologous type I angiotensin II receptors were present. A rat
genomic library was subsequently screened and four clones were isolated. From restriction
mapping, differential hybridization, polymerase chain reaction amplification and sequence
analyses we have determined that there are two unique type I angiotensin II receptor genes.
The first of these genes corresponds to the published rat vascular complementary DNA …
Abstract
A rat genomic Southern blot, probed with a type I angiotensin II receptor probe, demonstrated that two highly homologous type I angiotensin II receptors were present. A rat genomic library was subsequently screened and four clones were isolated. From restriction mapping, differential hybridization, polymerase chain reaction amplification and sequence analyses we have determined that there are two unique type I angiotensin II receptor genes. The first of these genes corresponds to the published rat vascular complementary DNA sequence; the second, corresponds to a novel receptor not previously described.
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